Sale! SAL Salbutamol Rapid Test Kit(Cassette)

SAL Salbutamol Rapid Test Kit(Cassette)

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Accuracy: >99%

Origin: China

Using: self-test

PRINCIPLE

The salbutamol test cassette is based on the principle of colloidal gold immunochromatography. An anti-salbutamol antibody is conjugated to colloidal gold and placed on conjugate pad. Colloidal gold provides red color to visualize antibody-antigen binding. Salbutamol antigen is immobilized on nitrocellulose membrane. After test sample is loaded onto sample pad, it mixes with gold-antibody conjugate and migrates along the membrane.

If the sample contains no salbutamol or amount of salbutamol lower than detection limit,

antibody conjugated to colloidal gold will bind the antigen immobilized on membrane, leading to clear red color presented on membrane detection line where the test antigen is immobilized (as negative result).

If salbutamol concentration is higher than detection limit in the test sample, it will bind gold-antibody conjugate and prevent its binding onto the antigen line on membrane. As a result, no color will be visible on detection line on membrane.

TEST PROCEDURE

Collect approx. 20 ml of urine sample using a clean centrifuge tube or other suitable container. Sample needs to be frozen if immediate testing is not possible. Refrigeration is recommended for short-term storage, and care must be taken to avoid microbial growth or contamination. Centrifuge sample when precipitation or turbidity is visible, and supernatant is used as test solution.water and mix well, followed by ultrasound treatment in 37 C ultrasonic water bath for 30 minutes (with mixing every 10 minutes). Equilibrate contents of the tube to room temperature, centrifuge at 4000 rpm for 5 minutes and collect supernatant as test solution.

Add 2 drops of test solution from a), b) or c) described above into sample loading well with a disposable dropper, and observe result in 8 to 15 minutes.

Test Result Interpretation

Test result is interpreted by observing test line and control line shown in result window.

Negative (-):

both test and control lines are present, and test line is equal to or darker than control line. This indicates salbutamol 

concentration in sample is lower than 3 ng/ml;

Positive (+):

control line is present, and test line is either absent or lighter than control line. This result indicates salbutamol

concentration is higher than 3 ng/ml in sample;

Invalid test:

no control line is present. Please repeat the test using a new test cassette following instructions on this user guide.