SAL Salbutamol Rapid Test Swine Urine

SAL Salbutamol Rapid Test Swine Urine

Company Introduction

We,Gemc Technology (Zhengzhou) Co.,Ltd,supply kinds of rapid test kits: fertility test kits (pregnancy test,ovulation test,menapause test),infectious disease test (hepatitis test,malaria test,chlamydia test,gonorrhea test,h.pylori test,toxoplasma test,dengue test, typhoid test...),tumor markers test (psa test,fob test,cea test,fob test),cardiac markers test (myoglobin test,tropponin I test,ck mb test), DOA drug of abuse test (mor,opi,mop,met,thc, mdma...), urinalysis reagent test strips (protein, glucose.....), alcohol test, vaginal ph test.

Introduction

The salbutamol test cassette is a lateral flow cassette test for rapid on-site or in-lab detection of residual salbutamol in urine,blood serum,and animal feed of livestock and poultry.

Assay sensitivity:10 ng/ml (10 ppb)

Principle

The salbutamol test cassette is based on the principle of colloidal gold immunochromatography.An anti-salbutamol antibody is conjugated to colloidal gold and placed on conjugate pad.Colloidal gold provides red color to visualize antibody-antigen binding. Salbutamol antigen is immobilized on nitrocellulose membrane.After test sample is loaded onto sample pad,it mixes with gold-antibody conjugate and migrates along the membrane.If the sample contains no salbutamol or amount of salbutamol lower than detection limit,antibody conjugated to colloidal gold will bind the antigen immobilized on membrane,leading to clear red color presented on membrane detection line where the test antigen is immobilized (as negative result).If salbutamol concentration is higher than detection limit in the test sample,it will bind gold-antibody conjugate and prevent its binding onto the antigen line on membrane. As a result,no color will be visible on detection line on membrane (indicating positive result).

Contents

l Test cassette

l Desiccant

l Dropper

l Manual

Precaution

1. Open the package upon request to avoid the test cassette being damped.
2. Use one cassette and one dropper for one test. Store the rest of the test cassette in dark at 8-250C environment and avoid being damped.
3. Avoid touching the membrane in the cassette.
4. Each package containing one test cassette and one drop is for one time use only. Please do not reuse.

Sample preparation

Urine:Use the urine sample directly and do not need to dilute (use urine sample which is back to room temperature)

Operation procedures

Collect approx. 20 ml of urine sample using a clean centrifuge tube or other suitable container. Sample needs to be frozen if immediate testing is not possible.Refrigeration is recommended for short-term storage, and care must be taken to avoid microbial growth or contamination.Centrifuge sample when precipitation or turbidity is visible,and supernatant is used as test solution.

water and mix well,followed by ultrasound treatment in 37 C ultrasonic water bath for 30 minutes (with mixing every 10 minutes).Equilibrate contents of the tube to room temperature,centrifuge at 4000 rpm for 5 minutes and collect supernatant as test solution.

Add 2 drops of test solution from a), b) or c) described above into sample loading well with a disposable dropper,and observe result in 8 to 15 minutes.

Test Result Interpretation

Test result is interpreted by observing test line and control line shown in result window.

Negative (-) both test and control lines are present,and test line is equal to or darker than control line. This indicates salbutamol concentration in sample is lower than 3 ng/ml;

Positive (+):

control line is present,and test line is either absent or lighter than control line.This result indicates salbutamol concentration is higher than 3 ng/ml in sample;

Invalid test:

no control line is present.Please repeat the test using a new test cassette

following instructions on this user guide.