Hydroxytyrosol is mainly used in skin care products, which can protect skin cells from ultraviolet rays, effectively maintain skin softness and elasticity, and achieve the effect of skin rejuvenation.
When applied to skin care products, olive leaf extract has protective-antioxidant and repairing effects, as follows:
1. Protection-Antioxidant effect-Maintain the viability of skin cells
Ultraviolet rays can cause the formation of free radicals, which can damage skin cells and even cell death in the morning. Assuming that skin cells are exposed to light, look at the changes in keratinocytes and fibroblasts-some are coated with pure olive leaf extract, others are not coated. After 24 hours, observe the condition of the cells and find that: coated with pure olives The cells of the leaf essence are preserved almost intact.
2. Protection-Antioxidant reaction
Ultraviolet rays can also cause the breakdown of cell membrane lipids. This kind of imagination is peroxidation. After culturing the skin fiber cells, some are coated with pure olive leaf extract, while others are not. Afterwards, fluorescence analysis measures the degree of peroxidation of a special substance secreted during the decomposition process. Experiments have proved that pure olive leaf extract can effectively organize peroxidation, thereby highly protecting fiber cells.
3. Repair-promote the metabolism of collagen protein-promote the synthesis of collagen protein
In the process of skin aging, the ability of collagen protein synthesis gradually declines, fiber cells can only produce a small amount of collagen protein. We measured the promotion of collagen synthesis in a cell culture environment. Some added pure olive leaf extract, while others did not. The amount of proline (a type of amino acid) was used as an index to measure the newly synthesized collagen protein. Experiments have proved that olive leaf extract can significantly promote the production of collagen protein by fibroblasts.
4. Anti-glycan reaction
The glycan reaction phenomenon is a reaction that occurs in the cell membrane. It will cause collagen reticulation, so that the collagen protein loses its original elasticity, and the skin appears sunken, that is, wrinkles. This phenomenon gradually increases with age. After 8 days of incubation in a 37C test tube, the derivative of the glycan reaction was followed by fluorescence analysis-formed between lysine and glucose-6-phosphate (ester)ase. This experiment was also carried out with the addition of pure olive leaf extract and without pure olive leaf extract, which proved its important role in anti-glycan reaction.
5.-Anti-gelatin enzymes
Gliamase is an enzyme produced by fiber cells under the influence of free radicals. Under ultraviolet radiation, keratinocytes will release some information, which stimulates fiber cells to produce glialase. Add pure olive leaf extract to the keratinocyte culture medium and place it under UVA and UVB irradiation. Samples were taken from the fiber cell culture medium before and after, and the results proved that the pure olive leaf extract inhibits the production of "information" in keratinocytes caused by ultraviolet rays, and indirectly reduces the secretion of collagenase.