Intended Use of Drugs Rapid Test Cup
The Drugs of Abuse Integrated Cup is a rapid visual immunoassay for the qualitative, presumptive detection of any combination of drugs of abuse in human urine specimens at the cut-off concentrations listed below:
Test | Calibrator | Cut-off (ng/mL) |
ACE | Acetaminophen | 5,000 |
AMP | Amphetamine | 1,000 |
AMP | Amphetamine | 300 |
BAR | Barbiturates | 300 |
BUP | Buprenorphine | 10 |
BZO | Benzodiazepines | 300 |
COC | Cocaine | 300 |
COT | Cotinine | 200 |
EDDP | EDDP | 100 |
FYL | Fentanyl | 200 |
KET | Ketamine | 1,000 |
MDMA | Ecstasy | 500 |
MET | Methamphetamine | 1,000 |
MET | Methamphetamine | 500 |
MTD | Methadone | 300 |
OPI/MOR | Morphine 300 | 300 |
OPI/MOR | Morphine 100 | 100 |
OPI2000 | Opiates 2000 | 2,000 |
OXY | Oxycodone | 100 |
PCP | Phencyclidine | 25 |
PPX | Propoxyphen | 300 |
TCA | Tricyclic Antidepressants | 1,000 |
THC | Marijuana | 50 |
TML | Tramadol | 100 |
Principle of Drugs Rapid Test Cup
The Multiple Drugs Test Cup is an immunoassay based on the principle of competitive binding. Drugs that may be present in the urine specimen compete against their respective drug conjugate for binding sites on their specific antibody.
During testing, a portion of the urine specimen migrates upward by capillary action. A drug, if present in the urine specimen below its cut-off concentration, will not saturate the binding sites of its specific antibody. The antibody will then react with the drug-protein conjugate and a visible colored line will appear in the test line region of the corresponding drug strip. The presence of drug above the cut-off concentration in the urine specimen will saturate all the binding sites of the antibody. Therefore, no colored line will form in the test line region.
A drug-positive urine specimen will not generate a colored line in the specific test line region of the strip because of drug competition, while a drug-negative urine specimen will generate a line in the test line region because of the absence of drug competition. To serve as a procedural control, a colored line will always appear at the control line region, indicating that proper volume of specimen has been added and membrane wicking has occurred.
Procedure and Result Reading of Drugs Rapid Test Cup
